Moss joined the Vancouver Children's Musical Theatre at eleven. She began working on television in 1989 and had recurring roles on the Dark Justice, Matrix, Models Inc. and F/X television series.
Moss had her major film break with The Matrix in 1999 and went on to play the hacker Trinity in the franchise's sequels The Matrix Reloaded and The Matrix Revolutions.
Other notable film roles include Memento for which she won an Independent Film Spirit award in 2002, Disturbia, Fido, Elephant Song and Snow Cake for which she won a Genie award in 2006.
Carrie-Anne Moss has been married to actor Steven Roy since 1999 with whom she has a daughter and two sons.